Influence of cerium on monoculture Microcystis aeruginosa (Kützing) Kützing
Keywords:
cerium, cyanobacteria, biomass, proteins, lipids, chlorophyll a, carotenoidsAbstract
The work is devoted to the study of the influence of cerium in the form of salt and nanoparticles on the culture of gram-negative toxic freshwater cyanobacteria Microcystis aeruginosa (Kützing) Kützing. M. aeruginosa is a typical representative of the algae flora of temperate latitudes, capable of producing neuro- and hepatotoxins (microcystin and cyanopeptolin). It should be noted that the species is a promising producer of organic raw materials for various needs: energy and biofertilizers, as well as a source of nutrients and biologically active substances. The effect of nanosized cerium dioxide (HP CeO2) and CeCl3 salt on the production parameters of cyanobacteria was evaluated. There was a significant increase in biomass in the culture of M. aeruginosa in response to the addition of cerium in both studied forms - HP CeO2 and CeCl3, although the increase in biomass is expressed differently depending on the applied concentrations (0.001-10 mm). High concentrations of cerium salt (10 mM) inhibited the growth activity of the test object, whereas when a similar amount of HP CeO2 was applied, the amount of M. aeruginosa biomass was maximum and at the end of the experiment was almost six times the amount of biomass of the control sample. It was found that the use of cerium does not affect the content of chlorophyll a in the cells of M. aeruginosa, while the lipid content in the cells of the studied cyanobacteria increases significantly: 2-2.5 times at concentrations of 0.01 - 1 mm, and at 0.001 mm lipid content 4 times higher than their content in control samples. Concentrations of 10 - 100 mm sharply (8 times compared to the number of lipids in the control sample) reduced the lipid content in M. aeruginosa cells. The use of HP CeO2 or CeCl3 is accompanied by a decrease in the total amount of proteins: revealed almost a tenfold decrease at concentrations of 10-100 mm; the maximum protein content was detected at 0.0001 mm HP CeO2, but this figure is 2.5 times less than the control sample. It was determined that the concentration of HP CeO2 10 mm can significantly increase the yield of biomass of M. aeruginosa; and the use of a concentration of 0.001 mm is accompanied by a maximum increase in lipid content and balanced levels of protein and chlorophyll a.
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