Activity of purine nucleotide catabolic enzymes in the liver of rats under conditions of nutritional imbalance
DOI:
https://doi.org/10.31861/biosystems2020.02.119Keywords:
animal model, liver, high-sucrose diet, low-protein-high-sugar diet, purine catabolismAbstract
The aim of the study was to investigate the activity of purine nucleotide catabolism enzymes, in particular, AMP-deaminase, 5'-nucleotidase, guanosine deaminase, and guanosine phosphorylase and xanthine oxidase in the cytosolic fraction of the liver of rats under conditions of different dietary supply of sucrose and dietary proteins. Enzyme activity was determined by photo colorimetric method: AMP-deaminase activity by the amount of ammonia formed by deamination of AMP, which has a maximum absorption at λ-540 nm and 5'-nucleotidase activity by the amount of Pn formed by hydrolysis of AMP at λ-8. The activity of guanosine phosphorylase, guanosine deaminase and xanthine oxidase was determined by spectrophotometric method. The results of studies have shown that due to consuming a high-sucrose diet in on the background of protein deficiency, the activation of purine nucleotide catabolism is observed and it can lead to disruption of the regulation of energy-dependent processes in liver cells. A critical factor influencing on the state of the purine nucleotide system and the activity of enzymes of their catabolism is alimentary protein deficiency.
References
Asadullina N. R., Gudkov S.V., Bruskov V. I. Antioxidant properties of xanthosine when exposed to X-rays. Basic research. 2011; 10: 22–25.
Voloshchuk O. M., Kopilchuk G. P. The state of the adenylyl nucleotide system in the liver of rats with toxic hepatitis under conditions of protein deficiency. Biophysics. 2017; 62 (6): 1–6.
Sumbaev V. V., Rozanov A.Y. Xanthine oxidase as a component of the system of generating reactive oxygen species. Ukr. biochemistry magazine. 1997; 69 (5): 198–201.
Tapbergenov S. O., Sovetov B. S., Tapbergenov A. T. Features of the effect of adenosine, AMP and hyperadrenaline on immune status, enzymes of purine nucleotide metabolism and antioxidant system. Biomedical chemistry. 2016; 62 (6): 645–649.
Tapbergenov S. O., Tapbergenova S. M. Diagnostic possibilities for determining the activity of serum adenylate deaminase. Laboratornoe delo. 1984; 2: 104–107.
Filanovskaya L. I., Vartanyan A. V. Enzymes of catabolic transformations of purine nucleotides of lymphocytes in norm and at a chronic lymphocytic leukemia. Vopr. honey. chemistry. 1985; 31 (3): 48–52.
Antonioli L., Blandizzi C., Pacher P., Hasko G. Immunity, inflammation and cancer: a leading role for adenosine. Nat. Rev. Cancer. 2013; 13: 842 – 857. https://doi.org/10.4103/njecp.njecp_2_16
Caliceti C., Calabria D., Roda A., Cicero A.F. Fructose Intake, Serum Uric Acid, and Cardiometabolic Disorders: A Critical Review. Nutrients. 2017; 9(4):387–395. https://doi.org/10.3390/nu9040395
Durga M., Gandham S. Biochemical characterization of kluyveromyces lactis adenine deaminase and guanine deaminase and their potential application in lowering purine content in beer. Front. Bioeng. Biotechnol. 2018; 6:1–10. https://doi.org/10.3389/fbioe.2018.00180
Esthervan D., Lucie A.G., Elianodos S., Joel J., Best L., Lennicke C., Vincent J., Marinos G., Foley A. Sugar-Induced Obesity and Insulin Resistance Are Uncoupled from Shortened Survival in Drosophila. Cell Metab. 2020; 31(4):710–725. https://doi.org/10.1016/j.cmet.2020.02.016
Fausther M. Extracellular adenosine: a critical signal in liver fibrosis. Am J Physiol Gastrointest Liver Physiol. 2018; 12 (19): 315. https://doi.org/10.1152/ajpgi.00006.2018
Kocic G., Nikolic J., Jevtovic-Stoimenov T., Sokolovic D. L-Arginine Intake Effect on Adenine Nucleotide Metabolism in Rat Parenchymal and Reproductive Tissues. The Scientific World Journal. 2012; 4 s. https://doi.org/10.1100/2012/208239
Lowry O., Rosebrough N., Farr A., Randall R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1): 75–265.
Maiuolo J., Oppedisano F., Gratteri S., Muscoli C. Regulation of uric acid metabolism and excretion. Vincenzo International Journal of Cardiology. 2016; 213: 8–14.
Michael K. McMullen Fructose Increases Uric Acid Contributing to Metabolic Syndrome – Herbal, Nutritional and Dietary Strategies to Reduce Uriс Acid. OBM Integrative and Complementary Medicine. 2018; 3(3):1–40. https://doi.org/10.21926/obm.icm.1803022
Rozhkovskij, J.V., Kresjun, V.I. Aktivnost’ markernyh fermentov i sostojanie lipidnogo matriksa membran jeritrocitov pri stresse i ego medikamentoznoj korrekcii. Ukrainian Journal of Biochemistry. 1991; 63(4): 74–80 (in Russian).
Srivastava R., Pinkoskу S., Filippov S., Hanselman J., Cramer C., Newton R. AMP-activated protein kinase: an emerging drug target to regulate imbalances in lipid and carbohydrate metabolism to treat cardio-metabolic diseases. J Lipid Res. 2012; 53(12):2490–2514. https://doi.org/10.1194/jlr.R025882
Tavenier M., Skladanowski A., De Abreu R. A., Willem J. Kinetics of adenilate metabolism in human and rat myocardium. Biochem. Biophis. Acta. 1995; 1244 (2-3): 351–356. https://doi.org/10.1016/0304-4165(95)98595-C
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