JOINT CULTIVATION OF REPRESENTATIVES OF THE GENERA PROPIONIBACTERIUM, LACTOBACILLUS, BIFIDOBACTERIUM ON UNIFIED MEDIA
DOI:
https://doi.org/10.31861/biosystems2023.01.032Keywords:
Propionibacterium, Lactobacillus, Bifidobacterium, associations, cocultivation, unified nutrient mediumAbstract
Probiotic microorganisms are living non-pathogenic, non-toxigenic microorganisms that enter the intestine with food and have a positive effect on the body, normalising the composition and biological activity of the microflora of the digestive tract. Lactic acid bacteria of the genera Lactobacillus and Bifidobacterium occupy a prominent place among the microorganisms that make up probiotics. The presence of these bacteria in the gastrointestinal tract provides an antagonistic environment that is directed against pathogenic microorganisms and creates conditions for the growth and development of beneficial bacteria. Bifidobacterium and Lactobacillus can enhance the absorption capacity of food components, lactose tolerance, stabilise liver function by normalising bowel function and reducing blood ammonia levels.
Recently, the spectrum of microorganisms that exhibit antagonistic properties and have a favourable effect on the vital activity of the organism has been expanding. Much attention is paid to propionic acid bacteria, which are part of the microflora of various biotopes. Propionic acid bacteria synthesise vitamin B12, bifidogenic growth factors and bacteriocins, have high adhesive and immunomodulatory properties, and their metabolites have antimutagenic effects. In this study, we analysed the possibility of individual and joint cultivation of representatives of the genera Propionibacterium, Lactobacillus and Bifidobacterium on unified media containing corn and yeast extracts. It was found that these nutrient media are effective for monocultivation of propionic acid bacteria and lactobacilli. At the same time, not only an increase in the optical density of propionic acid bacteria (3-fold), but also an increase in acid formation (2-fold on average) was observed. The possibility of joint cultivation of lactic acid and propionic acid bacteria by creating associations of two types was proved. The number of colonies of the studied microorganisms on selective nutrient media after cocultivation in unified media of both types was determined, the highest survival rate was observed for Propionibacterium. The antagonistic effect of both individual cultures and established associations against certain gram-positive bacteria and microscopic fungi was recorded.
References
Amara A. A., Shibl A. M. Role of Probiotics in health improvement, infection control and disease treatment and management. Journal of the Saudi Pharmaceutical Society. 2015; 23(2): 107–114. https://doi.org/10.1016/j.jsps.2013.07.001
de Vos W.M., Tilg H., Van Hul M., Cani P.D. Gut microbiome and health: mechanistic insights. Gut. 2022; 71(5): 1020-1032. https://doi.org/10.1136/gutjnl-2021-326789
Deutsch S-M., Mariadassou M., Nicolas P. et.al. Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study. Scientific Reports. 2017; 7: 46409. https://doi.org/10.1038/srep46409
Faye T., Holo H., Langsrud T. et.al. The unconventional antimicrobial peptides of the classical propionibacteria. Appl Microbiol Biotechnol. 2011; 89(3): 549-54. https://doi.org/10.1007/s00253-010-2967-7
Feng P., Ye Z., Kakade A et.al. A review on gut remediation of selected environmental contaminants: possible roles of probiotics and gut microbiota. Nutrients. 2018; 11(1): 22. https://doi.org/10.3390/nu11010022.
Frohnmeyer E., Deptula P., Nyman T.A. et.al. Secretome profiling of Propionibacterium freudenreichii reveals highly variable responses even among the closely related strains. Microb Biotechnol. 2018; 11(3): 510–526. https://doi.org/10.1111/1751-7915.13254
Fukuda S., Toh H., Hase K., Ohno H. Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature. 2011; 469(7331): 543–547. https://doi.org/10.1038/nature09646
Halttunen T., Collado M. C., El‐Nezami H., Meriluoto J., Salminen S. Combining strains of lactic acid bacteria may reduce their toxin and heavy metal removal efficiency from aqueous solution. Letters in applied microbiology. 2008; 46(2): 160-165. https://doi.org/10.1111/j.1472-765X.2007.02276.x
Hasan K.N., Banerjee G. Recent studies on probiotics as beneficial mediator in aquaculture: a review. The Journal of Basic and Applied Zoology. 2020; 81. https://doi.org/10.1186/s41936-020-00190-y
Hoseinifar S. H., Sun Y. Z., Wang A., Zhou Z. Probiotics as Means of Diseases Control in Aquaculture, a Review of Current Knowledge and Future Perspectives. Front Microbiol., 2018; 9: 2429. https://doi.org/10.3389/fmicb.2018.02429
Luna G.M., Quero G.M., Kokou F., Kormas K. Time to integrate biotechnological approaches into fish gut microbiome research. Curr Opin Biotechnol. 2022; 73: 121-127. https://doi.org/10.1016/j.copbio.2021.07.018
Massoud R., Zoghi A. Potential probiotic strains with heavy metals and mycotoxins bioremoval capacity for application in foodstuffs. J Appl Microbiol. 2022;133(3):1288-1307. https://doi.org/10.1111/jam.15685
Piwowarek K., Lipinska E., Hac-Szymanczuk E. et.al. Propionibacterium spp. – source of propionic acid, vitamin B12, and other metabolites important for the industry. Appl Microbiol Biotechnol. 2018; 102(2): 515–538. https://doi.org/10.1007/s00253-017-8616-7
Ruhal R., Kataria R., Choudhury B. Trends in bacterial trehalose metabolism and significant nodes of metabolic pathway in the direction of trehalose accumulation. Microbial Biotechnology. 2013; 6(5): 493–502 https://doi.org/10.1111/1751-7915.12029
Talwar C., Nagar S., Lal R., Negi R. K. Fish gut microbiome: current approaches and future perspectives. Indian J Microbiol. 2018; 58 (4): 397-414. https://doi.org/10.1007/s12088-018-0760-y
Taniguchi M., Nakazawa Н., Takeda O. Рroduction of a mixture of antimicrobial organic acids from lactose by co-culture of Bifidobacterium longum and Propionibacterium freudenreichii. Biosci Biotechnol Biochem. 1998; 62(8): 1522-1527. https://doi.org/10.1271/bbb.62.1522.
Wieërs G., Belkhir, L., Enaud R. et. al. How probiotics affect the microbiota. Frontiers in cellular and infection microbiology. 2020; 9: 454. https://doi.org/10.3389/fcimb.2019.00454
Wuertz S., Schroeder A., Wanka K. M. Probiotics in Fish Nutrition – Long-Standing Household Remedy or Native Nutraceuticals? Water. 2021. 13(10): 1348. https://doi.org/10.3390/w13101348
Zаrate G, Sаez G.D., Pеrez C. A. Dairy propionibacteria prevent the proliferative effect of plant lectins on SW480 cells and protect the metabolic activity of the intestinal microbiota in vitro. Anaerobe. 2017; 44: 58-65. https://doi.org/10.1016/j.anaerobe.2017.01.012.
